At Chem-Dry of Tampa FL we deal with extreme pet urine odors on a daily basis. Some of those jobs do require carpet pad removal and treating of the sub floor, but we do not recommend odor sealing the floor. There are two issues with sealing pet urine odors instead of removing them. The first issue is that the issue with bacteria is not addressed. The second issue is that the pet odor smell is not removed and it will eventually return.
We have come into many homes where there has been an attempt to remove the carpet pad and odor seal the sub floor and the odor has returned. If the pet urine odor is not neutralized then the problem doesn't go away. In some cases we are able to remove the pet urine odor without resorting to extreme measures and others require pressure washing of the sub floor combined with a urine treatment.
Pet urine odor removal can be tricky but with the right tools and the right solutions drastic measure can be avoided. We recommend hiring a professional urine odor removal service instead of going it alone.