Okay, so you've watched this video on how to remove cat urine from a mattress and your ready to go to work. Let me save you some time and effort. This method for removing urine from a mattress does not work. I'm a professional upholstery cleaner and I have removed urine from mattresses all over Tampa FL and I can tell you vinegar and baking soda does not eliminate or neutralize urine crystals in anything.
It used to be a very common practice, even by professional cleaners, to use a low pH (acidic) cleaner to remove urine for carpet and upholstery. Urine turns alkaline as it drys so acids can reduce the pH in the urine crystals but that doesn't remove it or even neutralize the odor. You have to use a cleaner that speeds up the natural decomposition process of the urine crystals to get rid of the urine stain.
This video does show you how to remove some of the surface urine out of a mattress, but most mattresses, especially tempurpedic ones, and are made like sponges. They have layers of batting to make them as soft as possible and they are not treated with any stain resistance so they are not water resistant. You can see how this might be a problem.
So how do you remove urine from a mattress? Well there is no easy answer. The best thing you can do is wet the area as quickly as possible. Douse the spot with water. I would recommend making it as hot as possible. You can just pour it straight onto the mattress and let it sit for about 30 seconds. Then you need to extract the moisture in a hurry. I recommend a wet dry vac or a spot remover vacuum. You can towel dry it as well. Use multiple towels soaking up as much moisture as possible and then moving to a clean dry towel to remove more. The more hot water you use the more urine you will remove. Now let it dry. If you live in Tampa FL you probably have a ceiling fan in your bedroom. Turn it on high and let it dry out completely. If you still have a urine stain and odor it's time to call a professional upholstery cleaner.